Addition of ___1___ causes the induction of the ___2___ polymerase. This polymerase then binds to the T7 ___3___ upstream of the rGFP gene, resulting in the ___4___ of rGFP gene to produce rGFP mRNA. This mRNA is then ___5___ to produce the rGFP protein.
Answers
The given question describes the process of the induction of polymerase and the production of mRNA. Here is the answer: Addition of "IPTG" causes the induction of the "T7" polymerase.
This polymerase then binds to the T7 "promoter" upstream of the rGFP gene, resulting in the "transcription" of rGFP gene to produce rGFP mRNA. This mRNA is then "translated" to produce the rGFP protein. What is IPTG?IPTG stands for isopropyl β-D-1-thiogalactopyranoside.
It is a molecular biology reagent used to induce the expression of genes under the control of the lac operon promoter. IPTG mimics the structure of allolactose, a natural inducer of the lac operon, and binds to the repressor of the lac operon, preventing it from binding to the operator region. This leads to the transcription of the gene(s) downstream of the promoter, which in this case is the T7 promoter.
The T7 promoter is a promoter used for the transcription of DNA sequences in plasmids into RNA. The promoter is a DNA sequence that initiates the transcription of a particular gene or DNA sequence. T7 promoter is derived from the T7 bacteriophage and is recognized and transcribed by T7 RNA polymerase.
T7 RNA polymerase is a highly efficient and specific enzyme that recognizes only the T7 promoter and transcribes the gene downstream of the promoter into RNA.What is mRNA?Messenger RNA (mRNA) is a type of RNA molecule that carries genetic information from DNA to ribosomes, where it is translated into proteins. mRNA is synthesized by the process of transcription in which DNA sequence is transcribed into mRNA. The mRNA sequence is then translated by ribosomes into proteins.
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Related Questions
The B-Vitamins in Red-Bull is what provides you with an IMMEDIATE boost of direct energy. Select one: O a. True. The ATP/CP energy system cannot generate ATP without B- Vitamins. O b. False. B-Vitamin
Answers
B-Vitamins, being a micronutrient, do not provide any immediate boost of direct energy as they do not contain usable energy. Option d. is correct.
B-Vitamins are essential micronutrients that play important roles in various metabolic processes in the body. While they are involved in energy metabolism and can support the conversion of nutrients into energy, B-Vitamins themselves do not directly provide an immediate boost of energy. The primary sources of immediate energy in Red Bull or any energy drink are typically caffeine and carbohydrates, not B-Vitamins. B-Vitamins are important for overall energy production and metabolism in the body but do not serve as an immediate source of energy.
Therefore, option d. "False. B-Vitamins are a micronutrient and do not contain any useable energy" is correct.
The complete question should be:
The B-Vitamins in Red-Bull is what provides you with an IMMEDIATE boost of direct energy. Select one:
a. True. The ATP/CP energy system cannot generate ATP without B- Vitamins.
b. False. B-Vitamins are the ONLY vitamin that does not contain useable energy.
c. True. B-Vitamins aid RBC and results in increase oxygen delivery
d. False. B-Vitamins are a micronutrient and do not contain any useable energy
e. Red Bull gives you wingsssssssssss
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select three reasons why mitosis is important
Answers
Answer:
Mitosis is important for three main reasons: development and growth cell replacement and asexual reproduction.
1. Development and growth. After meiosis has produced a gamete, and this has fused with another gamete to form an embryo, the embryo grows using mitosis. 2. Cell replacement. 3. Asexual reproduction.Explanation:
1. alveoli, tiny air sacs within the lungs muscle below the lungs used for, 2. bronchi, breathing the two tubes into which the, 3. diaphragm, trachea divides before entering the lung
Answers
Answer:
1. Alveoli: tiny air sacs within the lungs.
2. Bronchi: the two tubes into which the trachea divides before entering the lung.
3. Diaphragm: a muscle below the lungs used for breathing.
Explanation:
Living system can be defined as the internal systems of organisms and how materials circulate within organisms.
Some examples of living systems in organisms are respiratory system, nervous system, digestive system, and circulatory system.
Additionally, living systems comprises of the following components; cells, organs, muscle, tissues, blood, etc., which are typically used for carrying out various bodily functions such as respiration, metabolism, digestion, etc.
In Human anatomy, cardiac cycle can be defined as a complete heartbeat of the human heart which comprises of sequential alternating contraction and relaxation of the atria and ventricles, therefore causing blood to flow unidirectionally (one direction) throughout the human body.
1. Alveoli: it comprises tiny air sacs and it's located within the lungs of living organisms.
2. Bronchi: these are the two (2) tubes into which the trachea divides (the right bronchus and the left bronchus) before entering the lung.
3. Diaphragm: it's a muscle located below the lungs and typically used for breathing in all living organisms.
In an experiment, 90 newborn flies are divided evenly into three separate
groups and placed in boxes inside an incubator that is maintained at a
constant temperature of 30°C. On a daily basis, the first group is given water
containing 1 mg/mL of sugar; the second is given water containing 2 mg/mL
of sugar; and the third is given water containing 3 mg/mL of sugar. After four
weeks, the average length of the flies in each group is determined.
Which factor is the dependent variable in this experiment?
A. Average length of flies in a group
B. Concentration of sugar in the water
C. Number of flies in a group
D. Temperature of the incubator
Answers
Answer:
A. average length of files in a goup
can you help me with that please?
Answers
The most likely outcome resulting from the finches' beak structure adaptations is (option C) increased competition among finches.
What is adaptations?Adaptation refers to the process by which living organisms change or adjust their physical, behavioral, or physiological characteristics in response to changes in their environment, in order to improve their chances of survival and reproduction.
Adaptations can be structural, such as changes in an organism's anatomy, such as the shape of its beak or the color of its skin; functional, such as modifications in an organism's physiological processes, such as the ability to regulate body temperature or digest certain types of food; or behavioral, such as changes in an organism's actions or habits, such as migration patterns or hunting strategies.
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Particulates can:_____.
a. cause acid rain
b. reduce photosynthesis
c. cause lung and heart disease
d. none of these
Answers
Particulates can penetrate in respiratory system so the correct answer is c. cause lung and heart disease.
PM stands for particulate matter, often known as particle pollution, which is the word used to describe an airborne mixture of solid and liquid droplets. Dust, grime, soot, and smoke are examples of particulate that are large enough or dark enough to be visible to the unaided eye. Some can only be seen with an electron microscope because they are so tiny.
The lungs can be reached by particles in the PM2.5 size range that can penetrate far into the respiratory system. Shortness of breath, coughing, sneezing, runny nose, eye, nose, throat, and lung irritation are just a few short-term health impacts that can result from exposure to tiny particles.
Additionally, exposure to particulate can impair lung function and make illnesses like asthma and heart disease worse.
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The "three R's" describe an infant or toddler's
A. angry reactions to other children.
B. verbal responses to adults.
C. general interactions with others.
D. reaction to physical abuse by another child.
Answers
Answer:
C. general interactions with others.
Explanation:
The "three R's" refers to Respective, Responsive, Reciprocal. These three things are necessary for better growth and education of the child. The "three R's" are very important because the main focus of these "three R's" is to know the method that how the children learn different things. The teacher or parents should respectful while interact with the child. Being gentle is also a form of giving respect to the child. Always give response on the action made by the child.
True or false
Plants use energy from the sun to produce carbon dioxide and sugars???
Carbon dioxide is ether wrong or right
Answers
A scientist discovers a new molecule that is a long polymer that consists of a single strand of nucleotides. What type of molecule did the scientist most likely find?.
Answers
The big molecules that make up this polymer must have repeating units.
A polymer is made up of lengthy chains of monomers, which are smaller repeating units.
For instance, long chains of ethylene (CH2=CH2) molecules are connected end to end to form polyethylene.
Polyethylene's chemical formula is -CH2CH2-(CH2CH2)n-CH2CH2-, where n is typically a figure between 500 and 10,000.
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When there is decreased blood supply to the coronary arteries, which tissue of the heart is affected first?.
Answers
Narrowed arteries can obstruct blood flow to your heart muscle and the rest of your body, narrowed arteries can lead to chest pain. CAD can weaken the heart muscle over time.
The heart muscle receives blood from the coronary arteries. The heart muscle needs oxygen-rich blood in order to operate, just like all other bodily components do. Blood that lacks oxygen must also be transported away. Outside of the heart, the coronary arteries encircle the organ. Coronary artery disease may cause coronary blood flow to be decreased. As a result, there will be a greater extraction of oxygen from the coronary blood and less oxygen in the veins. Angina and tissue hypoxia result from this
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What solution is 2(x + 4) = 4x + 3 - 2x + 5
Answers
Answer:the answer is 8
Explanation:
2(x+4)= 8
4x+3-2x+5 also = 8
Check the organelles you think belong only in a
plant cell.
nucleus
chloroplasts
cell wall
Answers
Nucleus store dna
Chloroplasts conduct photosynthesis
Cell wall gives the plant structure
Pick one of these critical scarce resources:
• Phosphorus
• Scandium
• terbium
- Provide 3 reasons why this resource is so critical along with
what the resource is primarily used for.
- As the availability of the resource decreases, what would
you expect to happen to the price of the resource and
how will that impact the demand for the resource, and the costs of production of products that rely on that resource?
- Explain 3 ways the national economy would be impacted
by decreased availability of this resource.
- Explain 3 things American companies should do to
minimize the negative economic impacts of declining
availability of the resource.
- Explain 3 things the Federal government should do to
support the economy as this resource declines in
availability.
Answers
Let's focus on Phosphorus as the critical scarce resource. Here are the answers to your questions:
Reasons for Criticality and Primary Use of Phosphorus:
a. Essential Nutrient: Phosphorus is an essential nutrient for plant growth. It plays a crucial role in processes like photosynthesis, energy transfer, and DNA synthesis. Without phosphorus, plants cannot develop properly, impacting agricultural productivity.
b. Limited Reserves: Phosphorus reserves are finite and concentrated in a few countries. The scarcity arises from limited availability and geopolitical factors, making it critical for long-term sustainability.
c. Multiple Applications: Phosphorus is primarily used in fertilizers to enhance soil fertility and crop yields. It is also utilized in animal feed additives, detergents, food preservatives, and various industrial applications.
Impact of Decreased Availability on Price, Demand, and Production Costs:
As the availability of phosphorus decreases:
a. Price Increase: The price of phosphorus would likely rise due to supply-demand imbalances. Limited supply in the face of consistent or growing demand would drive up prices.
b. Demand Impact: Higher prices may lead to reduced demand for phosphorus-dependent products, especially in agriculture. Farmers might reduce fertilizer usage or seek alternatives, affecting the demand for phosphorus.
c. Production Costs: Industries relying on phosphorus would experience increased production costs due to higher input costs. This includes agricultural production, leading to potentially higher food prices, and other sectors dependent on phosphorus-based products.
Impact on National Economy:
Decreased availability of phosphorus can impact the national economy in several ways:
a. Agricultural Productivity: A decline in phosphorus availability would hamper agricultural productivity, leading to lower crop yields and potentially affecting food security.
b. Economic Sectors: Industries relying heavily on phosphorus, such as agriculture, food processing, and chemical manufacturing, may face challenges. The increased costs could impact their competitiveness and profitability.
c. International Trade: Countries heavily reliant on phosphorus imports might face trade imbalances and vulnerability in global markets. Dependence on a scarce resource increases the risk of supply disruptions and trade disputes.
Actions for American Companies:
American companies can minimize negative economic impacts by:
a. Resource Efficiency: Implementing technologies and practices that maximize the efficient use of phosphorus, such as precision agriculture and nutrient recycling systems.
b. Diversification of Inputs: Investing in research and development to identify and adopt alternative nutrient sources or develop phosphorus-efficient crop varieties.
c. International Cooperation: Collaborating with international partners to explore sustainable phosphorus management strategies, including recycling and responsible mining practices.
Actions for the Federal Government:
The Federal government can support the economy in the face of declining phosphorus availability by:
a. Research and Development: Investing in R&D initiatives to explore alternatives to phosphorus-based fertilizers, improve phosphorus recovery from waste streams, and promote sustainable agricultural practices.
b. Policy Measures: Implementing policies that incentivize phosphorus recycling, sustainable land management, and efficient fertilizer use. This can be done through regulations, tax incentives, or subsidies.
c. International Engagement: Engaging in global partnerships and collaborations to address phosphorus scarcity collectively, advocating for responsible mining practices, and ensuring fair trade in phosphorus-related products.
These actions aim to reduce dependence on phosphorus, promote resource efficiency, and stimulate innovation to mitigate the economic impacts of its declining availability.
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Which point shows the trade winds?
O 1
O 2
O 3
04
Answers
Long ago, the tree of life was seen as having five branches.
True or False?
Answers
Answer:
false, hopefully iss right
Please help me..
Please answer correctly
Answers
Answer:
See the procedure given in the attachment
1. Rocky Mountain spotted fever is a bacterial infection transmitted by a tick. What type of symbiosis occurs between a deer and the tick?
A. Mutualism
B. Commensalism
C. Parasitism
2. The oxpecker is a bird that eats the insects off the back of a zebra or rhino? What type of a symbiotic relationship is this?
A. Mutualism
B. Commensalism
C. Parasitism
3. Humans have “good” bacteria in their digestive tract, which assists in digesting food. The bacteria thrive there. What type of a symbiotic relationship is this?
A. Mutualism
B. Commensalism
C. Parasitism
4. Barnacles attach themselves to whales in order to be where food is available and also for transportation. This does not hurt the whale? What type of a symbiotic relationship is this?
A. Mutualism
B. Commensalism
C. Parasitism
Mutualism examples
Answers
Answer:
1. C
2. A
3. A
4. B
Explanation:
hope this helps. ♥️
Parasitism is the type of symbiosis that occurs between a deer and the tick. So, the correct option is C. This is because bacteria infect their host cell which is found in Deer.
What do you mean by Symbiosis?Symbiosis may be defined as a relative ecological association between the individuals of two or more different species.
The type of symbiotic relationship found between oxpecker and zebra is Mutualism. This is because oxpecker is able to get its prey (food), while zebra is benefitted from the removal of insects from their body. So, the correct option is A.
The type of symbiotic relationship between Humans and bacteria living in their digestive tract is Mutualism. This is because Humans are benefitted from bacteria as they assist in digesting food, while bacteria also benefit to find a proper place where unlimited food is available. So, the correct option is A.
The type of symbiotic relationship between Barnacles and whales is Commensalism. This is because Barnacles are benefitted from excess food and easy transportation, while whales are neither benefitted nor harmed by this relationship. So, the correct option is B.
Therefore, each of the questions are properly described above.
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The fluid in the bowman's capsule contains the same substances as blood plasma except it lacks?
Answers
The fluid in the bowman's capsule contains the same substances as blood plasma except it lacks proteins.
What is a bowman’s capsule?A part of the nephron called the Bowman's capsule forms a bag around the glomerulus that resembles a cup. The Bowman's capsule encloses the "Bowman's space," which marks the start of the urinary space and is situated next to the proximal convoluted tubule of the nephron. The subject of Bowman's capsule is British anatomist and surgeon Sir William Bowman (1816–1892). Prof. from the Russian Empire, a Ukrainian surgeon and anatomist, first described the entire microscopic anatomy of the kidney, including the nephrogenic capsule. Within it is the glomerulus, a cluster of microscopic capillaries where wastes are filtered from the blood.
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multicellular organisms must keep their cells awash in an "internal pond" because
Answers
Answer:
Multicellular organisms must keep their cells awash in an "internal pond" because cells that are not in contact with a liquid medium can quickly become dehydrated and lose the ability to exchange nutrients, gases, and waste products with their environment. Additionally, the internal liquid medium helps to maintain a stable internal environment, regulating the pH, temperature, and other important factors for proper cellular function.
Answer:
Multicellular organisms must keep their cells awash in an "internal pond" because:
* To transport nutrients and oxygen to the cells and remove waste products. The internal pond, or extracellular fluid, is a watery solution that surrounds all of the cells in the body. It contains dissolved nutrients, oxygen, and other essential molecules that are needed by the cells. It also contains waste products that need to be removed from the cells. The extracellular fluid is constantly being pumped around the body by the heart and circulated through the blood vessels. This helps to ensure that all of the cells in the body have a constant supply of nutrients and oxygen, and that waste products are quickly removed.
* To maintain a constant internal environment. The internal pond helps to maintain a constant internal environment for the cells. This is important because many of the chemical reactions that take place in the cells are very sensitive to changes in temperature, pH, and other factors. The extracellular fluid helps to buffer these changes and keep the internal environment stable.
* To protect the cells from injury. The extracellular fluid helps to protect the cells from injury by cushioning them from bumps and knocks. It also helps to prevent the cells from drying out.
* To provide a medium for cell growth and division. The extracellular fluid provides a medium for cell growth and division. It contains nutrients that are needed by the cells for growth, and it also helps to remove waste products that are produced by the cells.
The internal pond is an essential part of multicellular organisms. It helps to keep the cells healthy and functioning properly.
An organism that exhibits a head with sensory equipment and a brain probably also _____. A. is bilaterally symmetrical B. has a coelom C. is segmented D. is diploblastic
Answers
Option (A) is bilaterally symmetrical is correct.
An organism that exhibits a head with sensory equipment and a brain probably also is bilaterally symmetrical.
What is mean by bilateral symmetry?Symmetry where the same anatomical components are positioned on either side of a median axis, allowing for only one plane to split the subject into two nearly identical halves.Including Chordates, Arthropods, Molluscs, and Annelids.They are able to move ahead in a straight line because they have bodies that are bilaterally symmetrical (the same on both sides along an axis). Animals who could go from point A to point B swiftly and effectively would have been more successful in evolutionary history and would have been selected for over those that couldn't. The "head" and "tail" (anterior vs. posterior), front and back (dorsal vs. ventral), and right and left sides are all present in animals with bilateral symmetry. All real animals are bilaterally symmetrical, with the exception of those possessing radial symmetry.Learn more about symmetry here:
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What determines the direction of net movement of water across a cell membrane?
Answers
Answer: Osmosis
Explanation: Osmosis is the net movement of water across a selectively permeable membrane driven by a difference in solute concentrations on the two sides of the membrane.
Based on the pyramid, which organism probide the most available energy
Answers
Answer:
phytoplankton and bacteria
Explanation:
6. How could a dichotomous be helpful to you if you
were lost in the wilderness?
Answers
coyote
black-footed ferret
hawk
bison
rabbit
prairie dog
blue stem grass
Which argument for protecting the prairie dog best relates to the flow of energy in the ecosystem?
A. Prairie dogs eat grass and other plants that cattle graze on
B. Prairie dogs are an important source of food for many other species.
C Prairie dogs dig in the soil, which improves its quality and helps plants grow.
D. Prairie dogs make burrows which are used as habitat by other important species.
Answers
I think that would be B: Prairie dogs are an important source of food for many other species.
hope this helps. and please correct me if I'm wrong ^^
Is another name for the larynx and the importance it plays in the human body?.
Answers
The larynx in the human body is also called as Voice Box.
The larynx is a hollow tube which is connected to the pharynx, also called throat which is in turn connected to the whole body. Larynx helps to swallow smoothly and it also contains vocal chords. These vocal chords vibrate which helps humans to speak through voice created by the vibrations.
Furthermore, larynx is also involved in other functions such as breathing, producing sound and protecting the trachea. This larynx contains three parts in its cavity called as vestibule, the middle, and the infraglottic space. Vestibule is the upper portion of the cavity followed by the middle and infraglottic space.
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Look at the four devices below.
flashlight
lawn mower
solar calculator
desk lamp
Which device uses light as it's source on energy? ty if youhelp
Answers
every other option can be charged using batteries or power.
Which property of sodium
is not typical metal
,
Answers
Explanation:
It's a soft metal, reactive and with a low melting point, with a relative density of 0,97 at 20ºC (68ºF).
to what diameter must it be expanded to increase the blood flow by a factor of 5.0 with no change in pressure?
Answers
Increasing the radius of the vessel by a factor of the square root of 5 would increase blood flow by a factor of 5.0 while maintaining constant pressure.
The relationship between blood flow, pressure, and diameter of a vessel is complex and influenced by many factors, such as the viscosity of the blood, the elasticity of the vessel walls, and the resistance of the vessel to distension. However, in general, blood flow is directly proportional to the fourth power of the radius of a vessel. This is known as Poiseuille's law.
If we want to increase the blood flow by a factor of 5, we need to increase the radius of the vessel by a factor of the square root of 5.
The formula to calculate the diameter:
Diameter = (5^(1/4)) * Diameter_initial
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before pope gregory i was pope, he encountered a group of slave boys from _____________ whom he described as angels.
Answers
Before Pope Gregory I became Pope, he encountered a group of slave boys from Britain whom he described as angels.
Pope Gregory I, also known as Gregory the Great, encountered a group of slave boys from Britain before his papacy. He famously came across these boys in a slave market in Rome and was captivated by their appearance and demeanor. According to historical accounts, Pope Gregory I referred to them as "angels" due to their fair complexion and striking features.
This encounter with the slave boys from Britain had a profound impact on Pope Gregory I. It is said to have inspired him to send Christian missionaries to Britain to spread the teachings of Christianity. One of the missionaries sent by Pope Gregory I was Augustine of Canterbury, who played a crucial role in the Christianization of England.
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Gene on a chromosome lies close to another gene controlling a different trait. This indicates that jeans are blank the first gene contributes to the expression of the second gene. This indicates blank
Answers
Answer: genes are linked
Explanation: Gene on a chromosome lies close to another gene controlling a different trait. This indicates that genes are linked, meaning they are physically close to each other on the same chromosome. The proximity of these genes suggests that they are more likely to be inherited together as a unit, rather than independently assorting during the process of genetic recombination.