Which method is the best way to separate following two proteins, a: pi = 3.15, molecular weight 14kda, b: pi = 3.32, molecular weight 40kda?

Answers

Answer 1

The best method to separate the two proteins, a: pi = 3.15, molecular weight 14kDa, and b: pi = 3.32, molecular weight 40kDa is by SDS-PAGE electrophoresis.

SDS-PAGE electrophoresis (sodium dodecyl sulfate-polyacrylamide gel electrophoresis) is a popular analytical method for isolating proteins based on their molecular weight. This method helps to separate proteins from one another and also helps to determine the size of the protein that is being isolated.

SDS-PAGE involves the use of a negatively charged detergent called SDS to coat protein molecules, giving them a uniform negative charge that is proportional to their size. These negatively charged proteins are then separated by electrophoresis through a gel matrix, which is composed of polyacrylamide. The smaller the protein, the faster it migrates through the gel matrix.

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Related Questions

copious amounts of foul-smelling sputum are generally associated with

Answers

Copious amounts of foul-smelling sputum are generally associated with respiratory conditions that involve infection or inflammation of the airways.

One common cause is a bacterial infection such as bronchitis or pneumonia, where the excess sputum production is a result of the body's immune response to the infection. Foul odor in the sputum can indicate the presence of bacteria or other pathogens.

Other respiratory conditions such as lung abscess, bronchiectasis, or cystic fibrosis can also lead to an increased production of sputum with an unpleasant smell. These conditions often involve chronic inflammation and the accumulation of mucus in the airways, providing an environment for bacteria to grow.

In rare cases, foul-smelling sputum may be associated with conditions such as lung cancer or aspiration pneumonia, where the presence of foreign substances in the lungs can cause infection and the production of odorous sputum.

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What did Lamarck believe about the passing of traits to offspring? Explain why he was right/wrong?

Answers

Answer:

He believed traits changed/acquired through an indivual's lifetime could be passed down to offspring

Explanation:

This is basically evolution, living things aquire things that help them survive and pass it down to their offspring.

Question 8 of 10
A researcher wants to describe the effects of water pressure during a deep
ocean dive by comparing it to a column of water pressing down on an
aluminum can. Which type of model would best represent this?
OA. Mathematical model
OB. Physical model
OC. Conceptual model
OD. Computer model

Question 8 of 10A researcher wants to describe the effects of water pressure during a deepocean dive

Answers

Answer:

physical model

Explanation:

Answer:

C. conceptual model

What are functional groups? What are the names and structures of the most common functional groups? Write at least 10 common functional groups.

Answers

Functional groups are specific groupings of atoms or bonds within molecules that are responsible for the characteristic chemical reactions and properties of the compound. Functional groups are the center of reactivity in organic chemistry. The names and structures of the most common functional groups are as follows: Hydroxyl (-OH).

This group is found in alcohols and has a single oxygen atom bonded to a hydrogen atom, it is also polar, and hydrogen bonding occurs easily. The chemical formula of hydroxyl is -OH. Carbonyl (>C=O): This group is found in aldehydes and ketones and has a double bond between a carbon atom and an oxygen atom. The aldehyde carbonyl is located at the end of the carbon chain while the ketone carbonyl is located in the middle of the carbon chain.

The chemical formula of carbonyl is >C=O. Amine (-NH2): This group is found in amino acids, and it is polar and can act as a base due to the presence of nitrogen. It consists of a nitrogen atom attached to two hydrogen atoms. The chemical formula of amine is -NH2.Carboxyl (-COOH): This group is found in organic acids, and it is both polar and acidic. It consists of a carbon atom double-bonded to an oxygen atom and single-bonded to a hydroxyl (-OH) group.

The chemical formula of carboxyl is -COOH. Ester (-COO): This group is found in esters, and it is used in the production of perfumes, lubricants, and many other compounds. It consists of a carbon atom double-bonded to an oxygen atom and single-bonded to another oxygen atom. The chemical formula of ester is -COO. Aldehyde (-CHO): This group is found in organic compounds and is responsible for the aroma of cinnamon.

It consists of a carbon atom double-bonded to an oxygen atom and single-bonded to a hydrogen atom. The chemical formula of aldehyde is -CHO.Ketone (-CO-): This group is found in organic compounds and is responsible for the aroma of peppermint. It consists of a carbon atom double-bonded to an oxygen atom and single-bonded to two carbon atoms. The chemical formula of ketone is -CO-.Methyl (-CH3): This group is found in organic compounds and is responsible for the aroma of bananas. It consists of a carbon atom bonded to three hydrogen atoms.

The chemical formula of methyl is -CH3. Ethyl (-CH2CH3): This group is found in organic compounds and is responsible for the aroma of apples. It consists of a carbon atom bonded to two hydrogen atoms and one carbon atom. The chemical formula of ethyl is -CH2CH3. Phenyl (-C6H5): This group is found in organic compounds and is responsible for the aroma of lavender.

It consists of a benzene ring, which is a ring of six carbon atoms bonded to each other and with hydrogen atoms bonded to the carbons. The chemical formula of phenyl is -C6H5.These are some of the most common functional groups and there are many more that are found in organic chemistry.

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What is the science of naming and classifying living things?
(A) Species
(B) Classification
(C) Taxonomy
(D) Eukarya

Answers

Answer:

C. Taxonomy

Explanation:

Answer:

Taxonomy

Explanation:

Carolus Linnaeus, the Swedish botanical taxonomist who was the first person to formulate and adhere to a uniform system for defining and naming the world's plants and animals.

The oceans, lakes and rivers that cover 75% of Earth's surface are called the
A
lithosphere.
B
troposphere.
с
mesosphere.
D
hydrosphere.

Answers

Hydrosphere. The prefix hydro- means water.

Black coat is dominant over white coat in guinea pigs. A homo-zygous black pig is mated with another homo-zygous black pig.
What are the genotypes of the parents?

A.) bb and bb
B.) BB and BB
C.) Bb and Bb
D.) BB and bb

Answers

Answer:

BB and BB

Explanation:

Can someone help me out with this please :)

Can someone help me out with this please :)

Answers

Answer:

b. herbivore and primary consumer

c. omnivore and secondary consumer

d. top predator and tertiary consumer

Explanation:

Which letters are true about hydrogen?
A. A hydrogen bond is stronger than an ionic bond
B. The attraction between the hydrogen atom on one water molecule have oxygen atom on another water molecule is an example
C. A hydrogen bond stronger than a covalent bond
D. They are the strongest bonds that form between molecules

Answers

Answer B
Reason: Hydrogen is one of the strongest bonds but it is weaker than ionic and covalent bonds

Which data can be measured quantitatively?
speed of light
odor of gas
texture of a solid
color of a flame

Answers

Answer:

speed of light

Explanation:

i took the test

Data can be measured quantitatively is (a) speed of light. Option a

Which data can be measured quantitatively?

The speed of light can be measured using different scientific methods, like experiments with electromagnetic waves. The speed of light is usually described using units like meters per second or kilometers per second.

In comparison, smell, feeling, and appearance are properties that cannot be measured with numbers. These characteristics are based on personal opinions and are usually explained with words instead of numbers. For instance, the smell of gas can be described as strong or pleasant, but it cannot be given a number or measured accurately.

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HELP!!!!!! FIRST PERSON TO ANSWER CORRECTLY GETS BRAINLIEST!

The movement of tectonic plates in two different locations is shown below:

Which statement is most likely true?

An earthquake may in occur in location a and subduction occurs in location b

Subduction occurs in both locations

Subduction occurs in location a and a volcanic eruption may occur in location b

Sea floor spreading occurs in both locations

HELP!!!!!! FIRST PERSON TO ANSWER CORRECTLY GETS BRAINLIEST!The movement of tectonic plates in two different

Answers

Answer:

An earthquake may in occur in location a and subduction occurs in location b

The answer is c I hope this helps

ATP synthase changes ADP to ATP when.......................................... passes through it.

Answers

ATP synthase changes ADP into ATP when light energy passes through it. The light-dependent reactions supply the Calvin cycle with CO2 and ATP.

Hope it helps uh.

what is the difference between defining: a life vs alive vs a living organism?

Answers

Answer:

The term living thing refers to things that are now or once were alive. A non-living thing is anything that was never alive. In order for something to be classified as living, it must grow and develop, use energy, reproduce, be made of cells, respond to its environment, and adapt.

A medium that inhibits the growth of organisms other than the one being sought is termed a(n)
A. synthetic medium.
B. specific culture medium.
C. selective medium.
D. enrichment medium.

Answers

A medium that inhibits the growth of organisms other than the one being sought is termed a(n) option c) selective medium.

A selective medium is a type of culture medium that is specifically designed to inhibit the growth of all organisms except for the one that is being sought. This is achieved by adding certain chemicals or nutrients to the medium that will either prevent the growth of unwanted organisms or promote the growth of the desired organism.


Selective media are commonly used in microbiology to isolate specific types of bacteria or other microorganisms from a complex mixture. For example, if a researcher wants to isolate a particular strain of E. coli from a sample that also contains other types of bacteria, they might use a selective medium that contains a specific antibiotic that only kills the other bacteria and allows the E. coli to grow.


Overall, selective media are an important tool in microbiology research and can help scientists to better understand the properties and behaviors of different types of microorganisms.

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FILL IN THE BLANK. The mobile stage of the Cnidarian life cycle is the _____ stage.A. medusaB. instarC. polypD. tadpole

Answers

The mobile stage of the Cnidarian life cycle is the medusa stage. The medusa stage is the bell-shaped form of a jellyfish that swims freely in the water. The other stage in the Cnidarian life cycle is the polyp stage, which is usually stationary and attached to a surface. The tadpole stage is not a part of the Cnidarian life cycle, but rather a developmental stage of amphibians.
The mobile stage of the Cnidarian life cycle is the A. medusa stage. Cnidarians, such as jellyfish, have two main stages in their life cycle: the polyp stage, which is generally sessile or stationary, and the medusa stage, which is free-swimming and mobile. The medusa stage allows the Cnidarian to move and disperse in its environment. The other terms mentioned, instar and tadpole, are not relevant to the Cnidarian life cycle. Instar refers to the developmental stages of certain insects, while tadpole is the larval stage of amphibians like frogs.

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16
seaweed floating on the ocean surface is
(1 Point)
a) planktonic
b) nektonic
c) benthic

Answers

The answer is Nektonic

What can be said about the DNA analysis of closely related organisms.

Answers

Answer:

Now scientists can also analyze DNA to discover how closely organisms are related. Every living creature has DNA, which has a lot of inherited information about how the body builds itself. Scientists can compare the DNA of two organisms; the more similar the DNA, the more closely related the organisms.

What is the difference between poison and medicine? How can we use science to figure out the difference.
Will mark u brainliest.

Answers

Answer:

not sure how to go about this question so if I'm wrong please tell me

Explanation:

The difference between poison and medicine is that poison is used to harm to one's health whether its an animal or human while medicine is used to cure an illness or an injury. We can use science to figure out the difference by testing the substance on an animal or stem cells of a human scientists do this various times to be assured of its outcome.

A virus's ability to infect an animal cell depends primarily upon the
a) presence of receptor sites on the cell membrane.
b) type of viral nucleic acid.
c) host cell's ability to phagocytize viral particles.
d) enzymatic activity of a host cell.
e) presence of pili on the host cell wall.

Answers

A virus's ability to infect an animal cell primarily depends upon the presence of receptor sites on the cell membrane (A).

Viruses are specific to the host cells they can infect, and this specificity is due to the interaction between viral surface proteins and receptor molecules on the host cell membrane. When a virus encounters a suitable host cell, its surface proteins bind to specific receptor sites on the cell membrane, allowing the virus to enter the cell.

This process is essential for viral infection because it determines whether the virus can gain access to the host cell's machinery to replicate and produce new viral particles. The type of viral nucleic acid, the host cell's ability to phagocytize viral particles, the enzymatic activity of a host cell, and the presence of pili on the host cell wall are all factors that can influence viral replication and spread within the host organism. However, these factors are secondary to the presence of receptor sites on the cell membrane, as they come into play only after the virus has successfully entered the cell.

In summary, the presence of receptor sites on the cell membrane is the primary factor that determines a virus's ability to infect an animal cell, as it enables the specific binding and entry of the virus into the host cell. Hence, the correct answer is Option A.

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The technology of DNA typing had its beginnings in 1985 with the work of

Answers

Answer:

Explanation:

The technology of DNA typing, also known as DNA fingerprinting, had its beginnings in 1985 with the work of Sir Alec Jeffreys, a geneticist at the University of Leicester in England. Jeffreys was studying variations in DNA sequences among individuals, and discovered that certain regions of the DNA, known as minisatellites, had variable numbers of repeated segments. Jeffreys realized that these variable regions could be used to create a unique "fingerprint" for each individual, as the probability of two individuals having the same DNA fingerprint was very low.

This breakthrough led to the development of techniques for DNA profiling and forensic analysis, which have since become important tools in criminal investigations and paternity testing, among other applications.

What step of cell respiration produces the most ATP?

Answers

Answer:

i think the electron transport chain but dont quote me on this.

Explanation:

The Krebs cycle produces the CO2 that you breath out. This stage produces most of the energy ( 34 ATP molecules, compared to only 2 ATP for glycolysis and 2 ATP for Krebs cycle). The electron transport chain takes place in the mitochondria.

Define polar molecule or polarity.This is not a test question, could you please simplify the response to as few sentences as possible?

Answers

Polarity is caused by electronegativity. Electronegativity is the ability of an atom to attract electrons and when in a molecule there is a region of low electronegativity and a region of high electronegativity we say that the molecule is polar.

Terrestrial plants have a waxy covering produced by the dermal tissues called the cuticle. If this waxy covering was overproduced so that the stomata became clogged, what would happen to the plant?.

Answers

The plant's capacity to carry out photosynthesis would've been constrained since it would be unable to exchange oxygen with carbon dioxide.

The plant will not be able to exchange Oxygen for Carbon dioxide as well as the plant's ability to carry out the process of photosynthesis would also be limited, if the stomata get clogged due to overproduction of cuticle.

All apical portions of land plants have waxy cuticles to reduce water loss. Their major job is to make it possible for gases like oxygen, water vapor, including carbon dioxide to enter and exit the leaf quickly.

The question is incomplete. The complete question is:-

Terrestrial plants contain a waxy covering produced by the dermal tissues known as the cuticle. Suppose this waxy covering was overproduced in such a way that the stomata became clogged, then what would happen to the plant?

(A) The plant will not be able to exchange Oxygen for Carbon dioxide as well as the plant's ability to carry out the process of photosynthesis would also be limited.

(B) The sunlight would not be able to penetrate the plant's ground tissue cells through the thick dermal tissue and the plant's ability to carry out photosynthesis would be harmed.

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Select ALL of the following that are directly involved with making a protein

Ribosome

Cell Membrane

DNA

Cytoplasm

Answers

Cell Membranes,Ribosome, Cytoplasm are used to make protein

Explanation:

Proteins are formed in a condensation reaction when amino acid molecules join together and a water molecule is removed. The new bond formed in protein molecules where amino acids have joined (-CONH) is called an amide link or a peptide link.

hope it will help you

"Draw a pathway please with these included hjst a short
summary.
stimuli
hippocamous
cortex
amygdala
corticomedial nuclei
basal lateral nucleus
central nucleus
dorsal longitudinal fasiculus

Answers

The pathway can be noted as - Stimuli ⇒ Hippocampus ⇒ Cortex ⇒ Amygdala ⇒ Cortical Medial Nuclei ⇒ Basal Lateral Nucleus ⇒ Central Nucleus ⇒ Dorsal Longitudinal Fasciculus

Events or variables that cause a response or activate sensory receptors are referred to as stimuli. The brain's temporal lobe contains the hippocampus, a structure that aids in learning, memory development, and spatial orientation. It takes in information about stimuli and processes it. The cortex, is the brain's outermost layer. Higher-order cognitive processes like perception, attention, memory, and language processing are controlled by it. The amygdala is an almond-shaped structure tucked away in the temporal lobe of the brain. It is essential for understanding and controlling emotions, especially anger and fear. It gets information from the cortex and hippocampal regions.

Cortical Medial Nuclei are amygdala nuclei that receive information from the cortex and the hippocampus among other areas. They aid in the processing and integration of contextual and emotional data. The cortical medial nuclei send information to the basal lateral nucleus, which is a component of the amygdala. It contributes to the development and regulation of emotional memories.

The amygdala's central nucleus plays a role in the development of emotional reactions and orchestrates the autonomic and behavioural reactions connected to emotions. The amygdala, hypothalamus, and brainstem are all connected by a network of nerve fibres called the dorsal longitudinal fasciculus. It participates in the coordination and transmission of signals that control autonomic function and emotional reactions.

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Lisa is using a magnet to pick up various
objects. Which object is not attracted to her
magnet?
(A cobalt
(В iron
(C) nickel
(D) wood

Answers

Answer:

wood is not attracted to her magnet

The image below represents two waves X and Y, traveling to the same medium at the same speed how are the two waves different?

The image below represents two waves X and Y, traveling to the same medium at the same speed how are

Answers

Answer: D is right

Explanation:   It seems both waves have same wavelength.

Because speed = wavelength · frequency  (v = λf)  and

f = v/λ, so frequency is same.  Because period T = 1/ frequency, also periods are same.  Wave Y has higher amplidude and its energy is greater.

Answer:

D

Explanation:

just had it on study island

Many people try to eliminate fat from their diets. Which is one reason it is
necessary for humans to eat fat?

Answers

Fats are carbohydrates, which give the body energy. They also protect the organs and keep the body warm :)

Someone please help the questions are

State the name of
The liquid that is produced by the liver and stored by organ p

Organ p

Next question is

Explain what happens to ingested fat at r in fig 1.1 before chemical digestion occurs

Someone please help the questions areState the name of The liquid that is produced by the liver and stored

Answers

Answer:

Organ p is gallbladder

Answer for 1 question:Bile is the greenish-yellow fluid (consisting of waste products, cholesterol, and bile salts) that is secreted by the liver cells to perform 2 primary functions: To carry away waste.

Answer for sec.question:The first part of the small intestine. It connects to the stomach. The duodenum helps to further digest food coming from the stomach. It absorbs nutrients (vitamins, minerals, carbohydrates, fats, proteins) and water from food so they can be used by the body.

Bile grabs onto the fats, and the emulsifiers increase their surface area, making them easier for digestive enzymes to act on. Following this, enzymes break apart fatty acids. Lipase from the pancreas further digests fats into monoglycerides and fatty acids.

Explanation:

Hope I helped you

Answer:

organ p is gallbladder

Explanation:

Bile grabs onto the fats, and the emulsifiers increase their surface area, making them easier for digestive enzymes to act on. Following this, enzymes break apart fatty acids. Lipase from the pancreas further digests fats into monoglycerides and fatty acids.

Why is the sky blue?
Why does the moon appear in the daytime?
How much does the sky weigh?
How much does the Earth weigh?
How do airplanes stay in the air?
Why is water wet?
What makes a rainbow?
Why don't birds get electrocuted when they land on an electric wire?

Answers

Answer:

1: because the sea reflects up to the sky and that's why the sky is blue

Answer:

1. Because its the reflection off the ocean

2. Because its the moons reflection across the sky

3. Depends on the layer of the sky your talkin about

4. 5.972 × 10^24 kg

5. The wings catch the wind and the thrusters in the back help keep it up.

6. because its H2O and thats just how it is.

7. Because they have hollow legs which helps them not get eletrocuted because it just passes through their legs

Explanation:

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money became the most important material resource in the industrial society. true or false -a. refer to the original data. how much will net operating income increase (decrease) per month if the company uses higher-quality components that increase the variable expense by $2 per unit and increase unit sales by 10%. 2-b. should the higher-quality components be used? Question 1Expand the following binomial (2a 3e)4In the expression of (3x-2)10 , What is the:6th termCoefficient of the 4th termTerm with x3 An is calculated by subtracting the firm's costs from its total revenues, O accounting profit; excluding opportunity cost O accounting profit; including opportunity cost O economic profit; excluding opportunity cost O opportunity cost: including economic profit 1/5 1/5 1/5 perimeter triangle horeses aree to race over certain english meadow for a distance fo 8.44 furlongs.What is the race distance in rods and chains? Find the value of x which maximizes the following function: f(x) = a ln x + (n - a) In(1 - 0), for 0 < a Appeal of the compliance officer's findings is a ______ level process. group of answer choices 1 2 3 4 If 15.7 kg of Al2O3(s), 56.4 kg of NaOH(l), and 56.4 kg of HF(g) react completely, how many kilograms of cryolite will be produced While in Europe, if you drive 103 km per day, how much money would you spend on gas in one week if gas costs 1.10 euros per liter and your car's gas mileage is 35.0 mi/gal Correlation is useful only for a. measuring the strength of a linear relationship b. automatically calculating covariances c. assessing the weakness of a linear relationship d. measuring the strength of a nonlinear relationship e. conveying the same information in a simpler format than a scatterplot how many photons/s are contained in a beam of electromagnetic radiation of total power of 135 w when the each of the following is the source. (a) am radio station of 1240 khz photons/s (b) 7 nm x-rays photons/s (c) 4 mev gamma rays? photons/s After his Political Science class, Andre only remembered the parts of his professor's lecture that he agreed with. This is an example of selective there is no energy stored in the circuit at the time is switch is opened. a. derive the differential equation that governs the behavior of i2 (i.e. the right loop) if l1 g If the rate of population growth gl decreases, starting from the steady state, what happens in the short-run (few periods after the change) to the level of (a) Per-capita capital. (b) Per-capita output. (c) Per-capita consumption What happens when stimulus exceeds the threshold potential? The regulatory hormones that control the anterior pituitary gland arrive from the hypothalamus by way of the ______. Which describe a Mendelian trait najavits l., lung j., froias a., paull n., bailey g. a study of multiple behavioral addictions in a substance abuse sample. subst. use misuse. 2014;49:479484. doi: 10.3109/10826084.2013.858168 true or false medieval statues were often freestanding and rarely attached to buildings as decorations