to use tlc, it is necessary to be able to see where the spots are after eluting and drying the plate. which of the following are possible ways to see where the compounds are on the plate. most compounds make complexes when exposed to iodine vapor, and the iodine is intensely colored. some compounds are naturally colored, so you just need to look.

Answers

Answer 1

After eluting and drying the plate, it is vital to be able to view the spots in order to employ TLC.

What is TLC?

The chromatography method known as thin-layer chromatography can be used to separate non-volatile materials (TLC). Thin-layer chromatography is performed on a sheet of glass, plastic, or aluminium foil that has been coated with a thin coating of an adsorbent material—typically silica gel, aluminium oxide (alumina), or cellulose.

UV light is often used to quickly and non-destructively detect spots (compounds) on a TLC plate. Aromatic systems and strongly conjugated systems greatly absorb UV radiation. The majority of TLC plates contain zinc sulphide, which when exposed to short-wavelength UV makes them seem green.

When the TLC plate is put in the chamber and covered, iodine sublimes  with the compounds on the plate, resulting in yellow-brown blotches and interacts

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Related Questions

By adding SDS (sodium dodecyl sulfate) during the electrophoresis of proteins, it is possible to:
determine a protein's isoelectric point.
determine an enzyme's specific activity.
determine the amino acid composition of the protein.
preserve a protein's native structure and biological activity.

Answers

By adding SDS during electrophoresis of proteins, it is possible to determine the amino acid composition of the protein.

Sodium dodecyl sulfate (SDS) is an anionic detergent added during SDS-PAGE (SDS-polyacrylamide gel electrophoresis) to denature proteins and provide them with a uniform negative charge. This allows proteins to be separated based on their molecular weight, rather than their native structure or isoelectric point.

During electrophoresis, proteins migrate towards the anode due to the negative charge provided by SDS. By comparing the migration of the protein bands to a molecular weight standard, you can determine the amino acid composition of the protein. SDS does not preserve the protein's native structure or biological activity, nor does it allow for determination of isoelectric point or specific enzyme activity.

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At a certain temperature, 2.50 g Ca reacts completely in 30.0 seconds. The rate of consumption of Ca is

Answers

The rate of consumption of Ca is 0.0833 g/s.

The rate of consumption of Ca can be determined by dividing the mass of Ca consumed (2.50 g) by the time taken for the reaction to occur (30.0 seconds). This gives us a rate of 0.0833 g/s, indicating that 0.0833 grams of Ca are consumed every second during the reaction at the given temperature.

In chemical reactions, the rate of consumption or production of a substance is typically expressed in terms of the change in concentration over time. In this case, since the mass of Ca consumed is given, we can directly calculate the rate of consumption.

It's important to note that the rate of consumption of Ca may vary with temperature and other reaction conditions. The given rate applies specifically to the given temperature and the specific reaction conditions mentioned in the problem.

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Someone pls help me I will make you brain

Someone pls help me I will make you brain

Answers

Answer:

As shown in the attachment,

Explanation:

Someone pls help me I will make you brain

The flavoring is what seems to appeal to ______users and it’s what makes vaping seem _________

The flavoring is what seems to appeal to ______users and its what makes vaping seem _________

Answers

The flavoring is what seems to appeal to underage users and it’s what makes vaping seem harmless

Millions of years ago the Earth formed as a giant ball of molten rock. The outer surface
cooled forming a thin, solid outer crust. Volcanic activity on the surface produced an
atmosphere containing the compounds carbon dioxide, ammonia, methane and water
vapour.
Describe the bonding in any one of these compounds. You must include electronic
structures in your explanation.

Answers

The chemical bond between carbon and oxygen to form carbon dioxide involves sharing of electrons between the atoms and is known as a covalent bond.

What is chemical bonding?

A chemical bond is a link that holds two or more atoms together.

An enduring attraction between atoms or ions known as a chemical bond is what allows molecules and crystals to form. The bond may be created by the sharing of electrons in covalent bonds or by the electrostatic attraction of two oppositely charged ions, as in ionic bonds.

Covalent bonds are formed between electronegative elements that share electrons. These are usually atoms of the same or different non-metals. For example, the bond between carbon and oxygen to form carbon dioxide is a covalent bond.

Ionic bonds are formed between atoms of an electropositive element and atoms of an electronegative element. These are usually between metals ad non-metals.

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Calculate the number of moles water produced by the complete reaction of
109 g of hydrogen. 99

Answers

The number of moles water produced by the complete reaction of

109 g of hydrogen. 99 is given  below:

What is moles ?

Moles in chemistry are a unit of measurement used to quantify the amount of substance in a given sample. It is defined as the amount of a substance that contains the same number of particles as there are atoms in 12 grams of Carbon-12. This is known as Avogadro's number and is equal to 6.02 x 10^23. The mole allows scientists to calculate the mass, volume, and number of atoms or molecules in a given sample. It is a very useful tool for measuring the amount of a substance, as it takes into account the fact that different elements and compounds have different molecular weights. The mole is also used in equations to determine the mass, volume, and concentration of a substance, as well as the number of molecules in a given sample.

The equation for the complete reaction of hydrogen is:

2H2 + O2 → 2H2O

Since one mole of hydrogen reacts with one mole of oxygen to produce two moles of water, we can calculate the number of moles of water produced by the complete reaction of 109 g of hydrogen.

Using the molar mass of hydrogen (2.016 g/mol), we can calculate the number of moles of hydrogen present:

Moles of hydrogen = (109 g H2) / (2.016 g/mol H2)

Moles of hydrogen = 53.9 mol H2

Since one mole of hydrogen reacts with one mole of oxygen to produce two moles of water, the number of moles of water produced by the complete reaction of 109 g of hydrogen is:

Moles of water = (53.9 mol H2) x (2 mol H2O/1 mol H2)

Moles of water = 107.8 mol H2O

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let's consider that ethyl acetate is the ideal solvent for this tlc experiment. what might have happened to the spots if you had used t-butyl methyl ether as the eluent instead of ethyl acetate.

Answers

The spots will remain towards the bottom of the TLC plate in the TLC experiment if the eluent is changed from ethyl acetate to t-butyl methyl ether.

The spots will remain at the bottom of the TLC plate because t-butyl methyl ether is less polar and cannot overcome attractions of sample to adsorbent.

TLC experiment concept is simple. In TLC, adsorbent is silica which is highly polar. Then the eluent we can use as in different percentage of ethyl acetate & ether solution depending upon the polarity of given products.

As we know that polar molecules strongly bind with other polar molecules, ethyl acetate and silica gel are highly polar, the solvent ethyl acetate overcomes the strong bonding between the spotted product and adsorbent and takes the spot with the solvent upto the top.

If in this TLC experiment, instead of ethyl acetate, t-Butyl methyl ether is used, which is a highly non-polar or less polar solvent, it does not overcome the bonding since polar spots don't have greater binding affinity towards this non-polar ether solvent, therefore, the spots will remain tightly bonded with silica on TLC plate and spots will remain at the bottom of the TLC plate.

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A particle of matter that has no electrical charge and forms part of the nucleus of an atom is called: ________

Answers

Answer: The neutron

Explanation:

The neutron is a particle with no electrical charge and it resides in the nucleus of the atom.

(Look at picture) !!!!!!!
A particle of matter that has no electrical charge and forms part of the nucleus of an atom is called:

An enzyme's specificity can be due to: Group of answer choices amount of substrate available molecular recognition based on structural complementarity the ratio of catalyzed rate to the uncatalyzed rate of reaction. amount of enzyme produced by the cell.

Answers

Answer:

molecular recognition based on structural complementarity

Explanation:

Enzymes work on the basis of a lock and key model. The substrates on which enzymes act are specifically tailored to suit the shape of the enzyme.

As such, the enzyme just fits into the substrate in the same way as the right key fits into a lock.

Hence, molecular recognition based on structural complementarity is the basis for the specificity of enzyme activity.

The total mechanical energy of a particle is 73.4 J. At one instant, it has a kinetic energy of 55.7 J. If its mass is 3.00 kg, what is its distance above the ground at this instant

Answers

Its distance above the ground at that instant is approximately 0.600 meters.

To find the distance above the ground at this instant, we need to calculate the potential energy of the particle and then use the formula for potential energy to find the height. Here's the solution:

1. Total mechanical energy (E) = 73.4 J
2. Kinetic energy (KE) = 55.7 J

Since the total mechanical energy is the sum of kinetic and potential energies, we can find the potential energy (PE):

3. Potential energy (PE) = E - KE = 73.4 J - 55.7 J = 17.7 J

Now we can use the formula for potential energy to find the height (h):

4. PE = m * g * h, where m is the mass, g is the acceleration due to gravity (approximately 9.81 m/s²), and h is the height above the ground.

Rearranging the formula for height:

5. h = PE / (m * g) = 17.7 J / (3.00 kg * 9.81 m/s²) ≈ 0.600 m

So, the particle is approximately 0.600 meters above the ground at this instant.

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Thermal equilibrium occurs when two objects transfer temperatures.

Awnsers: True Or False

Answers

Answer:

False

Explanation:

Thermal equilibrium occurs when two objects transfer heat which is a form of energy, not temperature

Can someone help me thx asap

Can someone help me thx asap

Answers

Answer:

solvent

Explanation:

the solvent is usually a liquid substance

The substance that is a larger proportion of a solution and surrounds the solute to dissolve it is called the solvent.

How have humans influenced the Antarctic tundra ecosystem?

Humans have moved into the area and cleared out several plants and trees.

Humans have increased the greenhouse gasses found in the atmosphere.

Humans have adapted to live in colder areas and moved into the Antarctic tundra.

Humans have hunted several species that are only found in the Antarctic tund.

Answers

Answer: b

Explanation:

2. (10 Points) If something has a high heat capacity will it take a little energy or a
lot of energy to change its temperature? Defend your answer.

Answers

I uploaded the answer to a file hosting. Here's link:

tinyurl.com/wtjfavyw

Is the reaction between a chalk and hydrochloric acid always exothermic? How so?

Answers

The most part of Chalk is Calcium Carbonate (CaCO3). When this reacts with Hydrochloric acid, we have production of Carbon dioxide, Calcium Chloride and Water.

CaCO3 + 2HCl → CO2 + CaCl2 + H2O

What is the citric acid concentration in a soda if it requires 32.27 ml of 0.0148 m NaOH to titrate 25.00 ml of soda?

Answers

If it takes 32.27 ml of 0.0148 m NaOH to titrate 25.00 ml of soda, the citric acid concentration is 0.0191M.

The balanced chemical equation for the reaction between citric acid and NaOH is:

H3C6H5O7 (citric acid) + 3NaOH → Na3C6H5O7 (sodium citrate) + 3H2O

Citric acid concentration = (moles of NaOH) / (volume of soda in liters)

moles of NaOH = concentration of NaOH x volume of NaOH used in the titration

moles of NaOH = 0.0148 M x 0.03227 L = 0.000477796 moles

Now, we can calculate the citric acid concentration using the formula above:

Citric acid concentration = 0.000477796 moles / 0.025 L = 0.01911184 M

Therefore, the citric acid concentration in the soda is 0.0191 M (or 19.1 mM).

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23. Fatty acyl CoA desaturase is responsible for the synthesis of: a. Saturated fatty acid c. Strictly essential fatty acid b. Unsaturated fatty acid d. Glucogenic amino acid 24. Ketone bodies are: a. Water-soluble compounds produced as byproducts when fatty acids are broken down for energy in the liver (produced by liver mitochondria) and kidney 3

Answers

23. Correct option is b. Fatty acyl CoA desaturase is responsible for the synthesis of unsaturated fatty acid. 24. Correct option is a. Ketone bodies are water-soluble compounds produced as byproducts when fatty acids are broken down for energy in the liver and kidney.

Fatty acyl CoA desaturase is a vital enzyme that is responsible for the desaturation of fatty acyl CoA to unsaturated fatty acids. It helps to convert fatty acyl CoA molecules with long, saturated carbon chains into molecules with at least one double bond, producing fatty acids. The reaction is important for the biosynthesis of unsaturated fatty acids, which are important components of the cell membrane.

Saturated fatty acid is produced by enzymes such as stearoyl-CoA desaturase. Saturated fatty acids have no double bonds between the carbon atoms of the fatty acid chain, so they are saturated with hydrogen atoms. They are solid at room temperature and are abundant in dairy products, meats, and some oils.

Ketone bodies are water-soluble compounds produced as byproducts when fatty acids are broken down for energy in the liver and kidney. They are formed when the body uses fat instead of glucose as a source of energy, and they provide an alternative fuel for the brain and other organs. The liver can produce ketone bodies from fatty acids when glucose is scarce. This occurs during fasting or when a low-carbohydrate diet is followed. The three types of ketone bodies are acetone, acetoacetate, and β-hydroxybutyrate.

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a sample of nitrogen gas is confined to a balloon that has a volume of 1.88 l and a pressure of 1.334 atm. what will be the volume of the balloon if the pressure is changed to 0.662 atm? assume that the temperature and the amount of the gas remain constant.

Answers

The volume of the balloon is 3.788l.

Boyle's law:

Boyle's law state that the volume of a gas at constant temperature varies inversely with the pressure exerted on it.

The formula of Boyle's law:

P1V1 = P2 V2

P1 = 1.334atm

V1 = 1.88l

P2 = 0.662 atm

Putting the values in the equation we have:

1.334 × 1.88 = 0.662 × V2

V2 = 2.51/ 0.662

    = 3.788 l

Therefore the volume is 3.788l

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What mass of ammonia can be made from 20.0 g of hydrogen?
3H2 + N2 -> 2NH3

Answers

Answer:

20.0 g H2 x 1 mole H2/2g = 10.0 moles

moles NH3 = 10.0 mol H2 x 2 mol NH3/3 mol H2=6.67 moles

mass NH3 = 6.67 moles x 17g/mol = 113 grams

Coal and diamond are both minerals .they are both expensive.why

Answers

Answer:

hard to find

Explanation:

Answer:

because actually diamonds is coal.

Explanation:

how diamonds is coal? because when the coal gets very high pressure it turns into diamonds.

for a particular redox reaction, nono is oxidized to no−3no3− and fe3 fe3 is reduced to fe2 fe2 . complete and balance the equation for this reaction in basic solution. phases are optional.

Answers

Overall balanced equation for this reaction in basic solution is:2Nono + 6OH− + 3Fe3+ → 2NO3−NO3− + 3Fe2+Fe2+ + 3H2OH2O. The phases for the species involved in the reaction are optional.

The given redox reaction is:NONO is oxidized to NO3−NO3− and Fe3+Fe3+ is reduced to Fe2+Fe2+.This reaction can be represented in ionic form as:Nono + Fe3+ → NO3−NO3− + Fe2+Fe2+

We will now balance this redox reaction in basic solution using half-reaction method.Balancing the oxidation half-reaction:Nono → NO3−NO3−As we can see, the nitrogen atom is already balanced on both sides. The oxygen atoms are balanced by adding 3OH−OH− ions to the reactant side.The balanced oxidation half-reaction is:Nono + 3OH− → NO3−NO3− + 2H2OH2O + 2e−2e−Balancing the reduction half-reaction:Fe3+ → Fe2+Fe2+We can balance this half-reaction by adding two electrons to the product side.

The balanced reduction half-reaction is:Fe3+ + 2e− → Fe2+Fe2+Now, we will balance the number of electrons transferred in both half-reactions. To do this, we will multiply the oxidation half-reaction by 2.The balanced complete ionic equation is:2Nono + 6OH− + 3Fe3+ → 2NO3−NO3− + 3Fe2+Fe2+ + 3H2OH2O

The spectator ions are OH−OH− ions.

To get the net ionic equation, we will cancel out the spectator ions from both sides of the equation.The balanced net ionic equation is:2Nono + 3Fe3+ → 2NO3−NO3− + 3Fe2+Fe2+Overall balanced equation for this reaction in basic solution is:2Nono + 6OH− + 3Fe3+ → 2NO3−NO3− + 3Fe2+Fe2+ + 3H2OH2OThe phases for the species involved in the reaction are optional.

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What product is formed when each compound undergoes an intramolecular reaction in the presence of acid? H -NH₂ Z-I A A l dehuriae A and B readily cyclize to form hemiacetals. Draw the stereoisomers formed in this reaction from

Answers

The reaction of H-NH₂ Z-I A and A l dehuriae A in the presence of an acid leads to the formation of two stereoisomers of a cyclic hemiacetal, which contain both an alcohol and a carbonyl group in the same molecule.

The intramolecular reaction of H-NH₂ Z-I A and A l dehuriae A in the presence of an acid results in the formation of hemiacetals. Hemiacetals are cyclic compounds that contain both an alcohol (-OH) and a carbonyl (C=O) group in the same molecule.

To draw the stereoisomers formed in this reaction, we need to understand the structure of the reactants. H-NH₂ represents a primary amine (R-NH₂), which can react with a carbonyl group to form an imine (R-N=CR'). Z-I A and A l dehuriae A are aldehydes, which contain a carbonyl group (-CHO) at the end of the carbon chain.

When these compounds undergo an intramolecular reaction in the presence of acid, the carbonyl group of the aldehyde reacts with the amine group of the same molecule to form a cyclic hemiacetal.

The hemiacetal will have a stereocenter at the carbon atom that was part of the carbonyl group. The product will have two possible stereoisomers, depending on the orientation of the substituents around the stereocenter.

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Which type of plate boundary is associated with seafloor spreading at the source of the spread: convergent, divergent, or transform?

Answers

Answer:

The answer is divergent

Can you please help me to answer this?
I give you a brainlest! ​

Can you please help me to answer this? I give you a brainlest!

Answers

9 is B
6 is A
i’m not sure about the rest

Select the correct answer

Giving brainliest

Select the correct answerGiving brainliest

Answers

Answer:

I'm gonna say A and B

Explanation:

Not sure though

Hope I helped :)

they are located between each of the atrium and ventricle to prevent blood from flowing backwards, what is it?​

Answers

Valves, they push blood forward

a sample of water, h2o, has a mass of 24.50 g. calculate the number of water molecules in the sample.

Answers

Therefore, the number of water molecules in the given sample is approximately \(8.18 * 10^2^3\) molecules.

What is Avogadro's number?

To calculate the number of water molecules in the given sample, we need to use the concept of Avogadro's number and the molecular weight of water.

The molecular weight of water (H2O) is:

H = 1.008 u (atomic mass units)

O = 15.999 u (atomic mass units)

Molecular weight of H2O = (2 x 1.008 u) + 15.999 u = 18.015 u

Using the molecular weight of water, we can calculate the number of moles of water in the sample:

Number of moles = mass / molecular weight

Number of moles = 24.50 g / 18.015 g/mol

Number of moles = 1.359 mol

Now, using Avogadro's number (\(6.022 *10^2^3\) molecules/mol), we can calculate the number of water molecules in the sample:

Number of water molecules = Number of moles x Avogadro's number

Number of water molecules = \(1.359 mol * 6.022 *10^2^3\) molecules/mol

Number of water molecules =\(8.18 * 10^2^3\)molecules

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Can you answer my question in SCIENCE... Directions: Below are the reactants and products of photosynthesis. Choose the chemical symbol that matches the word.​

Can you answer my question in SCIENCE... Directions: Below are the reactants and products of photosynthesis.

Answers

Answer:

Water :H2O

Carbon dioxide :CO2

Oxygen :O2

Glocuse :C6H12O6

Answer:

water = H20

carbon dioxide = CO2

oxygen = 02

glucose= C6H1206

Explanation:

Ammonia (NH3) reacts with oxygen to form nitrogen monoxide and water. All the materials involved in this reaction are gasses. 0.100 moles of each of the reactants are initially introduced to a 5.0-liter reaction vessel. a. What would be the quantity of each gas in the container upon completion of the reaction? b.What would be the partial pressure of each gas on the reaction vessel upon reaction completion if the temperature of the system is 105 degrees C? c. What is the total pressure of all the gases on the reaction vessel at 105 degrees C?

Answers

Answer:

a. 0.02 moles  of NH₃, 0 moles of O₂, 0.08 moles of NO, 0.12 moles of H₂O

b. \(P_{NH_3}\) = 12,576.5 Pa, \(P_{NO}\) = 50,306.05 Pa, \(P_{H_2O}\) =  74,459.1 Pa

c. The total pressure is 138,341.64 Pa

Explanation:

a. NH₃ + O₂  → NO + H₂O

The balanced chemical equation is first found to be

4NH₃ + 5O₂  → 4NO + 6H₂O

Therefore, we have;

4 moles of NH₃ reacts with 5 moles of O₂ to form 4 moles of NO and 6 moles H₂O

Dividing by the reactant with the highest number of moles which is 5 moles of oxygen gives;

4/5 moles  of NH₃ reacts with 5/5 moles of O₂ to form 4/5 moles of NO and 6/5 moles H₂O

Which is the same as 4/5 moles  of NH₃ reacts with 1 mole of O₂ to form 4/5 moles of NO and 6/5 moles H₂O

Multiplying by 0.100 gives;

0.1×4/5 moles  of NH₃ reacts with 0.1 mole of O₂ to form 0.1×4/5 moles of NO and 0.1×6/5 moles H₂O

The quantity of each gas in the container upon completion of the reaction is therefore;

(0.1 - 0.1×4/5) = 0.02 moles  of NH₃

0 moles of O₂

0.08 moles of NO

0.12 moles H₂O

b. Given that the temperature = 105°C, we have;

PV = nRT

P = nRT/V

Where:

n = Total number of moles = 0.02 + 0.08 + 0.12 = 0.22 moles

R = Universal gas constant = 8.3145 J/(mol·K)

T = Temperature = 105°C = 378.15 K

V = Volume = 5 litre = 0.005 m³

P = 0.22×8.3145×378.15/0.005 = 138,341.64 Pa

From Dalton's law of partial pressure, we have;

Partial pressure Pₓ = Xₓ × P

Where:

Xₓ = Mole fraction

Which gives for ammonia NH₃ with 0.02 moles;

Mole fraction = 0.02/0.22 = 1/11

\(P_{NH_3}\) = 1/11 × 138,341.64  = 12,576.5 Pa

For the 0.08 moles of NO, we have

Mole fraction = 0.08/0.22 = 4/11

\(P_{NO}\) = 4/11 × 138,341.64  = 50,306.05 Pa

For the 0.12 moles H₂O

P = 0.12×8.3145×378.15/0.005 = 74,459.1 Pa

Mole fraction = 0.12/0.22 = 6/11

\(P_{H_2O}\) = 6/11 × 138,341.64  = 74,459.1 Pa

c. The total pressure = 12,576.5 Pa + 50,306.05 Pa + 74,459.1 Pa = 138,341.64 Pa.

Using only the groups of the periodic table, choose one element that is chemically similar to each of the following elements.

carbon
cesium
krypton
magnesium
aluminum
bromine

Answers

Elements that are in the same columns (vertical) are chemically similar.

Carbon- Si
Cesium- Na
Krypton- Ar
Magnesium- Ca
Al- try Boron
Br try Cl

These are just the ones I picked but it could be any in the same column
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