The enzyme that adds carbon to organic molecules during the dark phase of photosynthesis is called Rubisco (Ribulose-1,5-bisphosphate carboxylase/oxygenase).
Rubisco is essential for the Calvin cycle, which is the process by which carbon dioxide is fixed into organic molecules during photosynthesis. Without Rubisco, the plant would not be able to carry out the dark phase of photosynthesis and therefore would not be able to produce organic molecules necessary for its growth and survival. In the light-independent reactions, also known as the Calvin cycle, carbon dioxide (CO2) is fixed into organic molecules.
The first step of the Calvin cycle is the enzyme Rubisco adding CO2 to a five-carbon sugar called ribulose-1,5-bisphosphate (RuBP) to form a six-carbon molecule that is then broken down into two three-carbon molecules called 3-phosphoglycerate (3PG). These three-carbon molecules are then converted into a variety of organic molecules such as glucose, sucrose, and starch.
Rubisco is the key enzyme that catalyzes the addition of CO2 to RuBP, making it essential for the Calvin cycle and, therefore, for the synthesis of organic molecules during photosynthesis. If a plant is missing Rubisco, it would not be able to fix CO2 into organic molecules and, therefore, would not be able to carry out the light-independent reactions. This would result in the plant being unable to produce the organic molecules necessary for its growth and survival.
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what does hadean mean
Answer:
Hadean meaning: relating to or denoting the period when the earth was in the process of forming.
Explanation:
Hope this helps!
If not, I am sorry.
Answer:
Definition of Hadean
: of, relating to, or being the eon of history between the formation of the solar system and the formation of the first rocks on the earth — see Geologic Time Table.
Explanation:
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Deer that alert each other of predators is an example of a type mutualism called ___________.group of answer choices
Deer that alert each other of predators is an example of a type mutualism called alarm calling. Alarm calling is a kind of mutualism that falls under the broad category of defensive mutualism.
Mutualism is a form of interaction between two different organisms in which both organisms derive benefits. The relationship that develops as a result of mutualism is beneficial to both parties involved, and as a result, each party evolves traits that improve the relationship.
Therefore, the type of mutualism that is being referred to in this instance is alarm calling. Alarm calling occurs when an individual emits a warning call to alert other individuals of the presence of predators.
As a result of the warning call, the other individuals in the group can flee or take other defensive measures that reduce their chances of being caught by predators. In this manner, deer that alert each other of predators are engaging in mutualism by alerting each other of danger that can be harmful to them.
Therefore, deer that alert each other of predators is an example of a type mutualism called alarm calling and mutualism is a type of relationship that benefits both organisms.
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Write a paragraph telling where people who accept Christ as their Savior will spend eternity and why.
Answer:
Explanation:
according to the bible those who accept Christ after death shall go to heaven where they shall be with God this is because the kingdom of heaven shall be entered through the door which is Jesus and no one hall go to the father unless through the son
a new mutagen is able to cause frameshift mutations. which kind of mutagen would be capable of reversing this mutation?
It's important to note that the use of site-specific endonucleases for gene editing purposes is still an active area of research, and there are many technical challenges that need to be overcome before this approach can be applied to treating genetic diseases caused by frameshift mutations in humans.
A frameshift mutation occurs when a nucleotide is inserted or deleted from a DNA sequence, causing a shift in the reading frame of the genetic code. To reverse this type of mutation, a mutagen that can specifically remove or add the appropriate number of nucleotides would be required.
One type of mutagen that can achieve this is a site-specific endonuclease. Site-specific endonucleases are enzymes that can cleave DNA at specific sequences, such as restriction enzymes used in genetic engineering. By using a site-specific endonuclease that cuts at the site of the frameshift mutation, it may be possible to remove the extra nucleotides or add missing ones, effectively reversing the mutation.
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Which TORCH infection could be contracted by the infant because the mother owned a cat?
A. Toxoplasmosis
B. Varicella-zoster
C. Parvovirus B19
D. Rubella
The most appropriate answer in this context is A. Toxoplasmosis
The TORCH acronym stands for a group of infections that can be transmitted from a mother to her unborn baby during pregnancy. Each letter in the acronym represents a specific infection.
Toxoplasmosis is caused by the parasite Toxoplasma gondii, which can be found in cat feces.
If a pregnant woman comes into contact with the parasite through handling cat litter or contaminated soil, she can transmit the infection to her baby.
Varicella-zoster (B) is the virus that causes chickenpox and shingles. It is not directly associated with owning a cat.
Parvovirus B19 (C) is the virus responsible for fifth disease, a mild childhood illness. It is typically not related to cat ownership.
Rubella (D) is caused by the rubella virus. It is also not directly associated with cat ownership.
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This activity requires that you sort statements according to whether they are associated with transformation, conjugation, or transduction. Drag the statement to the gene transfer mechanism with which it is associated. A statement may be associated with more than one mechanism.
Transformation- This mechanism was first explored by Griffith as he studied smooth and rough strains of Streptococcus pnemoniae. Extracellular DNAses would block this mechanism.
What is transformation?Scientist Frederick Griffith conducted the first investigation into bacterial transformation in 1928. Griffith claims that the DNA or gene transfer from one type of bacteria to another can happen naturally or intentionally. For example, Transformation of non-virulent strain to a virulent cell or vice versa.
The process of transformation is when a competent bacterial cell (recipient's cell) takes up an extracellular or free DNA strand from one bacterial cell (donor's cell). Either naturally occurring processes or manmade processes are used to take up the DNA strand. The change occurs predominantly in the closely related species. Hence, transformation just refers to the direct insertion, integration and expression of the exogenous DNA in the competent bacterial cell.
Elaborating:Conjugation- This mechanism is initiated by cells with F plasmids.
This mechanism requires cell-to-cell contact. A chemical that blocked the formation of a pilus would block this genetic transfer mechanism.
Transduction- A virus that infects bacteria is a key component in this transfer mechanism.
All mechanisms- This mechanism may result in the transfer of genes that encode antibiotic resistance. This mechanism of genetic transfer always involves a one-way transfer from a donor cell to a recipient cell.
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Complete question:This activity requires that you sort statements according to whether they are associated with transformation, conjugation, transduction or all. Drag the statement to the gene transfer mechanism with which it is associated. A statement may be associated with more than one mechanism.
*This mechanism was first explored by Griffith as he studied smooth and rough strains of Streptococcus pneumonia
*Extracellular DNAses would block this transfer mechanism.
*This mechanism is initiated by cells with F plasmids.
*This mechanism may result in the transfer of genes that encode antibiotic resistance
*This mechanism requires cell-to-cell contact.
*A chemical that blocked the formation of a pilus would block this genetic transfer mechanism.
*This mechanism of genetic transfer always involves a one-way transfer from a donor cell to a recipient cell.
*A virus that infects bacteria is a key component in this transfer mechanism.
transformation
conjugation,
transduction
all mechanisms
How can a notochord differ from a proper
backbone?
Answer:
is that notochord is a flexible rodlike structure that forms the main support of the body in the lowest chordates; a primitive spine while backbone is the series of vertebrae, separated by disks, that encloses and protects the spinal cord, and runs down the middle of the back in vertebrate animals.
Explanation:
- Eijiro <3
What is the role of minerals to form a rock
Answer:
hm
Explanation:
Minerals are the building blocks of rocks. They are naturally occurring, inorganic solids that have a crystalline structure and a specific chemical composition. When minerals are combined through geological processes, they can form rocks.
The way in which minerals combine to form rocks depends on a variety of factors, including the chemical composition of the minerals, the temperature and pressure conditions, and the presence of water or other fluids.
For example, in the case of igneous rocks, which are formed from molten magma or lava, minerals crystallize and solidify as the magma or lava cools and hardens. As the magma cools, the minerals that require a higher temperature to form will crystallize first, followed by minerals that require lower temperatures.
In the case of sedimentary rocks, which are formed from the accumulation of sediment over time, minerals can be deposited along with other organic and inorganic materials. As these sediments are buried and compacted, the minerals become cemented together to form a solid rock.
In metamorphic rocks, which are formed from pre-existing rocks that have undergone intense heat and pressure, minerals can undergo a process of recrystallization or rearrangement to form new minerals and a new rock texture.
Overall, the type and arrangement of minerals in a rock can provide important information about the geological history and conditions under which the rock formed.
Answer:
Minerals are usually defined as an inorganic solid substances that are comprised of a specific chemical composition as well as well oriented and preferred internal structure of atoms. Some of the examples of minerals are calcite, quartz, olivine, pyroxene, gold, silver and many more.
There are numerous minerals present in earth, and they combine with one another and give rise to the formation of rocks. These minerals are present randomly in terms of oxides, sulfides, native elements.
Thus, rocks are considered to be the aggregate of minerals which are formed under different condition such as temperature, pressure, rate of cooling, sedimentation and compaction.
Explanation:
Minerals are made out of rocks
Compare and contrast viruses and Retrovirus
Answer:
the main difference between the two is how they replicate within a host cell.
which of the following contributes to antibiotic resistance?
a. giving antibiotics to livestock
b. taking multiple antibiotics at the same time
c. going to the doctor when an infection appears
Answer:
b since antibiotic resistance often results from overuse or underuse of medication
a is not possible because 'overuse' of antibiotics for livestock is not specifically mentioned but plainly 'giving'
c is also not possible because doctors can diagnose but not fall as an effect of resistance
The movement of genes from one population to another population is known as _____.
All answers are correct.
allele
gene pool
gene flow
The movement of genes from one group to another is referred to as gene flow.
The exchange of genetic information between populations is referred to as gene flow. This can occur through migration, where individuals move from one population to another and bring their genes with them, or through interbreeding between populations. Gene flow can have a significant impact on the genetic makeup of populations, introducing new alleles and changing the frequency of existing alleles in the gene pool.
Allele frequencies can change in local populations even while gene flow does not affect allele frequencies for a species as a whole. In the case of migration, the impact of the migrants on altering the genetic makeup of the resident population increases with both the number of migrants and the difference in allele frequencies between the resident and migrant populations.
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Which of the following statements is true about the Krebs (citric acid) cycle and the Calvin (light-independent) cycle?AThey both result in a net production of ATP and NADH.BThey both require a net input of ATP.CThey both result in a release of oxygen.DThey both take place within the cytoplasmic matrix.EThey both are carried out by enzymes located within an organelle matrix.
The statement which is true about the Krebs (citric acid) cycle and the Calvin cycle (light-independent) is: they both are carried out by enzymes located within an organelle matrix.
The Calvin cycle is a series of chemical events that converts carbon dioxide and hydrogen-carrier molecules into glucose. It is also referred to as the photosynthetic carbon reduction (PCR) cycle of photosynthesis, the dark reactions, the light-independent reactions, or the biosynthetic phase. Plants' stroma, the fluid-filled region of a chloroplast not shielded by thylakoid membranes, is where these processes take occur. These processes involve additional chemical steps that are applied to the byproducts of light-dependent reactions (ATP and NADPH). The Calvin cycle is present in all photosynthetic eukaryotes as well as a sizable number of photosynthetic bacteria.
The complete question is:
Which of the following statements is true about the Krebs (citric acid) cycle and the Calvin (light-independent) cycle?
A. They both result in a net production of ATP and NADH.
B. They both require a net input of ATP.
C. They both result in a release of oxygen.
D. They both take place within the cytoplasmic matrix.
E. They both are carried out by enzymes located within an organelle matrix.
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Which type of bond forms disaccharides?
A. 1-4 Hydroxyl bond
B. 1-5 Galactosidic bond
C. Hydrogen bond
D. 1-4 Glycosidic bond
Answer: D
Explanation:
Disaccharides type of bond forms 1-4 Glycosidic bond
Drinking alcoholic beverages on hot days is not safe because alcohol inhibits the release of ______ which normally helps to conserve water during dehydration.
A) antidiuretic hormone
B) oxytocin
C) thyroxine
D) triiodothyronie
Drinking alcoholic beverages on hot days is not safe because alcohol inhibits the release of the antidiuretic hormone which normally helps to conserve water during dehydration. Therefore, the correct answer is option A.
Antidiuretic hormone, also known as vasopressin, is a hormone that helps regulate water balance in the body by promoting the reabsorption of water in the kidneys. When ADH levels are high, the kidneys reabsorb more water, leading to a decrease in urine production and an increase in blood volume. However, when alcohol is consumed, it inhibits the release of ADH from the pituitary gland. This leads to a decrease in the reabsorption of water in the kidneys, resulting in an increase in urine production.
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If the mass of an object increases, what also increases
Answer:
The force does too
Explanation:
From the universal law of gravitation, F ∝ m1m. Since force is directly proportional to mass, if the mass of either of the object increases, the force also increases.
Liz is examining a plant cell under a microscope. She sees many small green strutures inside the cell. Her teacher explains that the process of photosynthesis takes place inside these structures. What type of structure is Liz looking at?
Answer:
Explanation:
Cell wall or cell membrane
Answer:
the correct answer is chloroplast
Veterinary Science!!
After going on a hunting trip where he and his buddies ran across a dead deer, Darius develops itchy bumps on his
hand. His friends tell him that he shouldn't have rolled the deer over, but he wanted to get a closer look. Now the
bumps have black spots in the middle. Concerned, Darius sees a doctor, who diagnoses him with anthrax poisoning.
Which treatment will Darius's doctor MOST likely prescribe?
antibiotic
anti-fungal
antiviral
anti-inflammatory
The treatment that Darius's doctor will MOST likely prescribe for anthrax poisoning is an antibiotic.
What is to be prescribed?Bacillus anthracis is the bacterium that causes anthrax, which can be treated with antibiotics like penicillin, doxycycline, or ciprofloxacin.
When the bacterium penetrates the skin by a cut or scratch, it causes itchy pimples with black spots in the middle, which is a sign of cutaneous anthrax, the most frequent type of anthrax. Anti-fungal, antiviral, and anti-inflammatory medications would not be effective in treating anthrax
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Alice was participating in a photosynthesis lab for science class. The lab procedures instructed Alice to prepare a test tube containing water, a sprig of elodea (a common aquarium plant), and 10 g of sodium bicarbonate (NaHCO3). She was then instructed to place the test tube in front of a strong light source for one minute. Alice noticed tiny bubbles forming on the leaves of the elodea. What is the most logical explanation for why Alice saw bubbles forming on the leaves of the elodea?Group of answer choicesThe bubbles formed as a result of the hydrogen from the sodium bicarbonate mixture dissolving the elodea leaves.The bubbles formed from the release of water molecules from the elodea leaves.The bubbles formed as the elodea plant released oxygen from the elodea leaves.The bubbles formed as the elodea plant released carbon dioxide from the elodea leaves.
Step 1.
Write down the experiment data.
- tube containing water;
- alodea (photosynthetic organism);
- 10 g of sodium bicarbonate;
- light source.
Step 2.
Why bubbles are formed on the leaves of the alodea?
The experiment have all the condictions for photosynthesis, since it provides: water (that is in the tube), CO2 (because of the dissolved sodium bicarbonate), light (because of the string light source). Therefore the most ogical explanation for Alice experiment results is: the bubbles formed as the elodea plant releases oxygen from the elodea leaves, that because with all the elements in the experiment the plant can do a normal photosynthetic process that way realing oxygen in the tube and forming bubbles of "air".
What are the good fats for your brain?
O the glycemic fats
O the omega fats
O low cholesterol
O high cholesterol
Answer:
B
Explanation: The omega fats, I hope it helps
a collection of cloned dna fragments representing the organism's entire genome is called a
A collection of cloned DNA fragments representing the organism's entire genome is called a genomic library.
A genomic library is a collection of cloned DNA fragments that together represent the entirety of an organism's genome, which can be used for various genetic studies, such as gene mapping, sequencing, and expression analysis.
The genomic library is created by isolating and breaking down the DNA from the organism's genome into smaller fragments using restriction enzymes, which are enzymes that cleave DNA at specific nucleotide sequences.
The fragments are then ligated into a vector, which is a DNA molecule that is used to carry the DNA fragments into host cells, and the vector is introduced into a bacterial or yeast host cell for cloning. The resulting library contains thousands to millions of bacterial colonies, each containing a cloned fragment of the genome.
The genomic library can be screened using various techniques to identify specific genes or DNA sequences of interest. For example, a probe can be designed that is complementary to a particular gene or DNA sequence, and the library can be screened using this probe to identify clones that contain the target sequence.
Overall, the genomic library is an essential tool in genetic research, allowing researchers to study the structure and function of genes and genomes in various organisms. It can provide insights into disease-causing mutations, genetic diversity, and evolutionary relationships between species.
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in one type of signaling an initial stimulus activates an enzyme to phosphorylate another enzyme, which in turn phosphorylates another enzyme. this process amplifies the signal from even just one activated receptor to many molecules with changed activity. this description most closely matches signaling by
The description most closely matches signaling by a kinase cascade.
A kinase cascade is a series of protein kinases that phosphorylate and activate each other in a sequential manner, amplifying the signal from an initial stimulus.
In a kinase cascade, the initial stimulus activates a receptor, which then activates the first enzyme, typically a protein kinase. This kinase phosphorylates and activates the next enzyme in the cascade, which in turn phosphorylates and activates another enzyme.
This process continues, amplifying the signal and resulting in the activation of many molecules with changed activity. This type of signaling allows for a rapid and amplified response to a specific stimulus, enabling cells to effectively respond to their environment.
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Match the name of the greenhouse gas with one of the human activities that produces it.
carbon dioxide
industrial processes
fluorinated gases
agricultural and industrial activities
methane
production and transport of oil and natural
gas
nitrous oxide
burning fossil fuels
Answer:
carbon dioxide- burning of fossil fuels
fluorinated gases- industrial processes
methane- production and transport of oil and natural
gas
nitrous oxide- agricultural and industrial activities
Explanation:
Greenhouse gases are gaseous components that induces greenhouse effect. This means that due to their structural ability to trap heat energy in the atmosphere, they contribute to the global warming effect on the Earth. However, this greenhouse gases are produced in large amounts as a result of the day-to-day anthropogenic activities. The following are the greenhouse gases and how they are produced.
Carbondioxide (CO2)- Carbondioxide is the most common and important greenhouse gas. It is generated via natural processes like respiration but also produced when fossil fuels e.g coal, petroleum etc. are burned.
Fluorinated gases- This is another greenhouse gas caused by activities of man. Fluorinated gases are produced in industries during the production of certain products.
Methane- Methane is a gaseous hydrocarbon and the principal constituent of natural gas. Therefore, the production and transportation of oil and natural gases can lead to the escape of methane into the atmosphere.
Nitrous oxide- Nitrous oxide is an oxide of nitrogen. It is mainly produced via agricultural processes such as nitrification and denitrification or use of fertilizers etc. and also industrial actions.
Answer:answer in pic
Explanation:
which glands secrete hormones that regulate metabolism of carbohydrates, proteins, and fats? select all that apply. one, some, or all responses may be correct. pancreas
The pancreas secretes hormones that regulate metabolism of carbohydrates, proteins, and fats.
The hormones insulin and glucagon are secreted by the pancreas and help regulate blood sugar levels and appetite, stimulate stomach acids, and tell your stomach when to empty.
Insulin and glucagon are two hormones secreted by the pancreas that are essential to regulating metabolism. Insulin helps the body absorb glucose from the bloodstream, while glucagon helps regulate glucose levels in the body. Insulin helps cells take in glucose, which is then converted into energy for the body. Glucagon helps the body break down stored energy, such as fat and glycogen, when glucose levels are low.
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Which of the following is an accessory organ of digestion?(a) stomach(b) pancreas(c) spleen(d) colon(e) esophagus.
The pancreas is an accessory organ of digestion.
The accessory organs of digestion are the pancreas, liver, and gallbladder.
The digestive system is the body system that processes food. It is made up of a series of organs that work together to break down food, absorb nutrients, and eliminate waste. The digestive system begins with the mouth, where food is chewed and mixed with saliva.
The food then travels down the esophagus to the stomach, where it is mixed with stomach acid and enzymes to begin the process of breaking down the food into nutrients that can be absorbed by the body.
Once the food is broken down, it moves into the small intestine, where it is further broken down and nutrients are absorbed into the bloodstream. Any remaining waste is then passed into the large intestine, where water is absorbed and the waste is formed into feces before being eliminated from the body through the rectum and anus.
The pancreas, liver, and gallbladder are accessory organs that play a role in digestion. The pancreas produces enzymes that help break down proteins, carbohydrates, and fats in the small intestine. The liver produces bile, which is stored in the gallbladder and helps break down fats. The gallbladder releases bile into the small intestine when needed.
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a cell with 12 pg in the nucleus during prophase 1 of meiosis 1. how many will be present in the nucleus of a haploid gamete?
a) 12 pg
b) 24 pg
c) 6 pg
d) 8 pg
The nucleus of a haploid gamete will have 6 pg during prophase 1 of meiosis 1. The answer is c.
During prophase 1 of meiosis 1, the cell undergoes DNA replication, resulting in duplicated chromosomes. Each chromosome consists of two sister chromatids, so the total amount of DNA in the nucleus is doubled. In this case, the cell initially had 12 pg of DNA in the nucleus during prophase 1.
In meiosis, there are two rounds of cell division, resulting in the production of haploid gametes. During the first division (meiosis 1), homologous chromosomes separate, reducing the chromosome number by half. However, the DNA content remains the same because the sister chromatids are still attached.
In the second division (meiosis 2), the sister chromatids separate, resulting in four haploid daughter cells. Each daughter cell receives half of the DNA content present in the initial cell. Therefore, in a haploid gamete, the amount of DNA in the nucleus will be half of the initial amount, which is 6 pg in this case. Therefore, the correct answer is C.
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describe the three stages of germination
Answer:
Germination is the process by which the seed embryo begins growth. A seed is considered to have germinated when the embryonic root emerges from the seed coat. ... Three major stages in the germination process are the imbibition of water, increased metabolic activity, and swelling of cells.
Answer:
The three stages in the germination are the imbibition of water, increased metabolic activity, and swelling of cells.
Explanation:
the flexor muscles in the anterior arm (biceps brachii and brachialis) are innervated by what nerve? a. musculocutaneous b. median c. radial d. ulnar
The flexor muscles in the anterior arm, specifically the biceps brachii and brachialis, are primarily innervated by the musculocutaneous nerve. Option A is correct.
The musculocutaneous nerve arises from the brachial plexus, a network of nerves originating from the spinal cord in the neck region. It travels down the arm and supplies motor innervation to the flexor muscles in the anterior compartment of the arm, including the biceps brachii and brachialis. Additionally, the musculocutaneous nerve provides sensory innervation to the skin on the lateral side of the forearm.
While the other nerves listed (median, radial, ulnar) also contribute to the innervation of the arm, they primarily supply different muscles or regions. The median nerve innervates muscles in the forearm and hand, the radial nerve innervates extensor muscles of the arm and forearm, and the ulnar nerve innervates muscles in the hand and provides sensory innervation to parts of the hand and little finger.
Hence, A. is the correct option.
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Which of these mutations do you think would have the worst effect on the function of
the protein?
an insertion or
deletion near
the end of a
gene
an insertion or
deletion at the
beginning of
an insertion at
the middle of
a nonsense
mutation at the
end of the gene
a missense
mutation
the gene
the gene
Answer:
Both are harmful for the organism in case of point mutation. In case of deletion or insertion of a particular fragment of the gene, deletion could be more harmful than insertion, because the deleted fragment of the gene will never be replaced at exact size and exact position of the disabled gene.
Explanation:
hope it helps
Las proteínas son sustancias utilizadas para la regeneración de los tejidos. Una célula que presente dificultades para producirlas debe tener algún tipo de alteración en
Answer:
Regeneracion tisular
Explanation:
Las celulas se encargan de muchas funciones, la principal es la regeracion tisular. Un ejemplo claro de esto es la formacion de colageno por parte del fibroblasto,
La regeneracion celular esta guiada por factores de crecimiento y estructuras proteicas que confieren resistencia y sosten ademas de elasticidad y soporte tisular.
What parts of the supply chain are most closely involved with
the situation in this case? What is the responsibility of each part
in order to maintain a smooth flow of material?
The parts of the supply chain that are most closely involved with the situation in this case are the suppliers, the manufacturer, the distributor, and the retailer. The responsibility of each part in order to maintain a smooth flow of material is as follows:
Suppliers: The suppliers are responsible for providing the manufacturer with high-quality raw materials on time. The suppliers are also responsible for ensuring that the raw materials are of the required specifications and quality.
Manufacturer: The manufacturer is responsible for converting raw materials into finished products. The manufacturer should ensure that they produce the required products on time and that they meet the desired quality standards. They should also ensure that they are using the right machinery and equipment to produce the required products.
Distributor: The distributor is responsible for transporting the finished products from the manufacturer to the retailer. The distributor should ensure that the products are delivered on time and that they are in good condition.
Retailer: The retailer is responsible for selling the finished products to the consumers. The retailer should ensure that they have the right products in stock and that they are selling them at the right price. They should also ensure that they are providing excellent customer service to their customers.
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